个人简介
刘铭汉，副教授，副主任医师，硕士生导师，入选中国科协青年托举人才工程（军事科技领域），重庆英才·青年拔尖人才。中华医学会细菌感染与耐药分会全国青年委员，重庆医师协会骨科微创学组常务委员.
学习与工作经历.
2008.09-2016.06，陆军军医大学，获得临床医学博士学位（本博连读）
2016.07-2021.11，陆军军医大学第二附属医院骨科，讲师，主治医师
2021.12-至今，陆军军医大学第二附属医院骨科，副教授，副主任医师
学术成果简介
在国内外重要刊物如Bioactive Materials, Stem Cells,Stem Cell Research & Therapy等发表SCI论文21篇，发表论文共被他人引用1200余次；授权专利3件；编写著作或教材2部。作为项目骨干获得2019年教育部高等学校科学研究科技进步一等奖1项。已指导多名研究生优异成绩毕业，其中获评重庆市优秀硕士论文1名。
研究领域
长期从事脊柱退行性疾病的临床诊治及研究，聚焦“椎间盘退变机制及干预策略”这一科学问题，开展椎间盘退变的精准诊疗及再生修复研究。通过谱系示踪、单细胞测序等技术探索椎间盘退变中干细胞在内源性修复中的作用及其分子机制，并利用高分子水凝胶、纳米酶等再生医学手段对椎间盘实施早期干预、精准修复。
承担的主要项目
[1]工程化外泌体促椎间盘修复机制及应用研究，中央军委科学技术委员会，2022.11-2025.11，120万，主持。
[2]重庆英才·青年拔尖人才项目，重庆市委组织部，2023.1-2016.12，40万，主持。
[3]骨桥蛋白(OPN)促进软骨终板干细胞向髓核内归巢及其力生物学机制研究，国家自然基金委员会，2018.01-2021.12,21万，主持。
[4]PGP激活CXCR1/2促进软骨终板干细胞向髓核内归巢并诱导其向促炎症
表型转化的机制研究，国家自然基金委员会，2016.01-2019.12,61万，项目骨干。
[5]基质弹性调控软骨终板干细胞生物学特性及其在构建组织工程髓核中的应用，国家自然基金委员会，2015.01-2019.12,61万，项目骨干。
[6]GATA4介导自噬抑制椎间盘内高氧引起髓核细胞衰老的作用和机制研究，国家自然基金委员会，2019.01-2022.12,61万，项目骨干。
[7]椎间盘髓核退变产物PGP促进软骨终板干细胞向髓核迁移的细胞力学机制研究，陆军军医大学，2021.01-2023.12,15万，主持。
[8]战创伤止血抗菌促修复敷料的制备及其作用研究，陆军军医大学，2020.01-2023.12,30万，主持。
[9]学科人才建设专项（学科优才），陆军军医大学第二附属医院，2023.01-2026.12,50万，主持。
代表性成果
[1]论文:
1、Luo L, Gong J, Wang Z, Liu Y, Cao J, Qin J, Zuo R, Zhang H, Wang S, Zhao P, Yang D, Zhang M, Wang Y, Zhang J, Zhou Y, Li C, Ni B, Tian Z, Liu M. Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration. Bioact Mater. 2022 Sep;15:29-43. pii: S2452-199X(21)00571-5. doi: 10.1016/j.bioactmat.2021.12.007. PubMed PMID: 35386360.
2、Luo L, Gong J, Zhang H, Qin J, Li C, Zhang J, Tang Y, Zhang Y, Chen J, Zhou Y, Tian Z, Liu Y, Liu M. Cartilage Endplate Stem Cells Transdifferentiate Into Nucleus Pulposus Cells via Autocrine Exosomes. Front Cell Dev Biol. 2021;9:648201. doi: 10.3389/fcell.2021.648201. PubMed PMID: 33748142.
3、Luo L, Jian X, Sun H, Qin J, Wang Y, Zhang J, Shen Z, Yang D, Li C, Zhao P, Liu M, Tian Z, Zhou Y. Cartilage endplate stem cells inhibit intervertebral disc degeneration by releasing exosomes to nucleus pulposus cells to activate Akt/autophagy. Stem Cells. 2021 Apr;39(4):467-481. doi: 10.1002/stem.3322. PubMed PMID: 33459443.
4、Cheng Z, Xue C, Liu M, Cheng Z, Tian G, Li M, Xue R, Yao X, Zhang Y, Luo Z. Injectable microenvironment-responsive hydrogels with redox-activatable supramolecular prodrugs mediate ferroptosis-immunotherapy for postoperative tumor treatment. Acta Biomater. 2023 Aug 4. pii: S1742-7061(23)00455-5. doi: 10.1016/j.actbio.2023.08.002. PubMed PMID: 37544392.
5、Zhou J, Yuan C, Liu C, Liu M, Zhou L, Wang J. Assessment of vertebral bone mineral density and stand-alone oblique lumbar interbody fusion for adjacent segment disease and primary lumbar degenerative diseases. J Orthop Surg (Hong Kong). 2022 Jan-Apr;30(1):10225536221091846. doi: 10.1177/10225536221091846. PubMed PMID: 35410526.
6、Wang Y, Yang Y, Zuo R, Wu J, Zhang C, Li C, Liu M, Zhou Y. FOXO3 protects nucleus pulposus cells against apoptosis under nutrient deficiency via autophagy. Biochem Biophys Res Commun. 2020 Apr 9;524(3):756-763. pii: S0006-291X(20)30253-9. doi: 10.1016/j.bbrc.2020.01.168. PubMed PMID: 32035615.
7、Sun C, Lan W, Li B, Zuo R, Xing H, Liu M, Li J, Yao Y, Wu J, Tang Y, Liu H, Zhou Y. Glucose regulates tissue-specific chondro-osteogenic differentiation of human cartilage endplate stem cells via O-GlcNAcylation of Sox9 and Runx2. Stem Cell Res Ther. 2019 Nov 28;10(1):357. pii: 10.1186/s13287-019-1440-5. doi: 10.1186/s13287-019-1440-5. PubMed PMID: 31779679.
8、Wang Y, Zuo R, Wang Z, Luo L, Wu J, Zhang C, Liu M, Shi C, Zhou Y. Kinsenoside ameliorates intervertebral disc degeneration through the activation of AKT-ERK1/2-Nrf2 signaling pathway. Aging (Albany NY). 2019 Sep 23;11(18):7961-7977. pii: 102302. doi: 10.18632/aging.102302. PubMed PMID: 31546235.
9、Li B, Sun C, Sun J, Yang MH, Zuo R, Liu C, Lan WR, Liu MH, Huang B, Zhou Y. Autophagy mediates serum starvation-induced quiescence in nucleus pulposus stem cells by the regulation of P27. Stem Cell Res Ther. 2019 Apr 15;10(1):118. pii: 10.1186/s13287-019-1219-8. doi: 10.1186/s13287-019-1219-8. PubMed PMID: 30987681.
10、Zuo R, Wang Y, Li J, Wu J, Wang W, Li B, Sun C, Wang Z, Shi C, Zhou Y, Liu M, Zhang C. Rapamycin Induced Autophagy Inhibits Inflammation-Mediated Endplate Degeneration by Enhancing Nrf2/Keap1 Signaling of Cartilage Endplate Stem Cells. Stem Cells. 2019 Jun;37(6):828-840. doi: 10.1002/stem.2999. PubMed PMID: 30840341.
11、Zuo R, Liu M, Wang Y, Li J, Wang W, Wu J, Sun C, Li B, Wang Z, Lan W, Zhang C, Shi C, Zhou Y. BM-MSC-derived exosomes alleviate radiation-induced bone loss by restoring the function of recipient BM-MSCs and activating Wnt/β-catenin signaling. Stem Cell Res Ther. 2019 Jan 15;10(1):30. pii: 10.1186/s13287-018-1121-9. doi: 10.1186/s13287-018-1121-9. PubMed PMID: 30646958.
12、Feng C, Liu M, Fan X, Yang M, Liu H, Zhou Y. Intermittent cyclic mechanical tension altered the microRNA expression profile of human cartilage endplate chondrocytes. Mol Med Rep. 2018 Apr;17(4):5238-5246. doi: 10.3892/mmr.2018.8517. PubMed PMID: 29393457.
13、Yao Y, Zhang H, Liu M, Liu H, Chu T, Tang Y, Zhou Y. Prognostic Factors for Recovery of Patients After Surgery for Thoracic Spinal Tuberculosis. World Neurosurg. 2017 Sep;105:327-331. pii: S1878-8750(17)30869-0. doi: 10.1016/j.wneu.2017.05.167. PubMed PMID: 28602882.
14、Liu MH, Cui YH, Zhou Y. Cellular mechanical properties reflect the differentiation potential of nucleus pulposus-derived progenitor cells. Am J Transl Res. 2016;8(10):4446-4454. PubMed PMID: 27830029.
15、Liu MH, Fu WJ, Cui YH, Guo QN, Zhou Y. Downregulation of Semaphorin-3F is associated with poor prognostic significance in osteosarcoma patients. Am J Cancer Res. 2016;6(10):2252-2262. PubMed PMID: 27822415.
16、Liu MH, Fu WJ, Cui YH, Guo QN, Zhou Y. Downregulation of Semaphorin-3F is associated with poor prognostic significance in osteosarcoma patients. Am J Cancer Res. 2016;6(10):2252-2262. PubMed PMID: 27822415.
17、Liu MH, Cui YH, Guo QN, Zhou Y. Elevated ASCL2 expression is associated with metastasis of osteosarcoma and predicts poor prognosis of the patients. Am J Cancer Res. 2016;6(6):1431-40. PubMed PMID: 27429855.
18、Liu MH, Bian BS, Cui X, Liu LT, Liu H, Huang B, Cui YH, Bian XW, Zhou Y. Mesenchymal stem cells regulate mechanical properties of human degenerated nucleus pulposus cells through SDF-1/CXCR4/AKT axis. Biochim Biophys Acta. 2016 Aug;1863(8):1961-8. pii: S0167-4889(16)30138-0. doi: 10.1016/j.bbamcr.2016.05.004. PubMed PMID: 27163878.
19、Liu MH, Sun C, Yao Y, Fan X, Liu H, Cui YH, Bian XW, Huang B, Zhou Y. Matrix stiffness promotes cartilage endplate chondrocyte calcification in disc degeneration via miR-20a targeting ANKH expression. Sci Rep. 2016 May 4;6:25401. pii: srep25401. doi: 10.1038/srep25401. PubMed PMID: 27142968.
20、Sun C, Shang J, Yao Y, Yin X, Liu M, Liu H, Zhou Y. O-GlcNAcylation: a bridge between glucose and cell differentiation. J Cell Mol Med. 2016 May;20(5):769-81. doi: 10.1111/jcmm.12807. PubMed PMID: 26929182.
21、Wang H, Zhou Y, Huang B, Liu LT, Liu MH, Wang J, Li CQ, Zhang ZF, Chu TW, Xiong CJ. Utilization of stem cells in alginate for nucleus pulposus tissue engineering. Tissue Eng Part A. 2014 Mar;20(5-6):908-20. doi: 10.1089/ten.TEA.2012.0703. PubMed PMID: 24102374.
专利:
含生长因子的椎间盘组织支架及其制备方法，国家发明专利,ZL201410087968.2(2016)。
永生化人软骨终板干细胞系的制备方法及其用途，国家发明专利，ZL201410088040.6 (2016)
一种大鼠软骨退变诱导纤维环及髓核退变模型及应用，国家发明专利，ZL201911192595.4 (2022)
基于全息影像的骨科康复训练互动系统V1.0，软件著作权证书，2021SR0286450 (2020)
[3]获奖：腰椎间盘退变性疾病微创外科创新治疗技术的建立与临床应用，教育部科技进步一等奖，2019。
联系方式
电话：15823512096；E-mail：liuminghan311@qq.com；
